Barking Owls have declined rapidly throughout much of their range. In Victoria it is estimated that there are only 50 pairs left.
Habitat loss and degradation is a major threat to the survival of the Barking Owl. Loss of hollow-bearing trees and firewood harvesting impacts on the species by removing nesting and roost sites as well as habitat for hollow-dependent prey such as gliders, possums and parrots.
Competition from feral honeybees for roost sites has been named a key threatening process for this species in NSW. Competition with foxes and feral cats, as well as predation by foxes is also thought to be a reason for their decline.
Barking Owl mortality has also been recorded due to secondary agricultural poisoning, barbed wire fences and vehicle collisions.