Male Yellow-throated Honeyeaters hold territories year-round, aggressively defending them against others of their own species as well as other birds and particularly, other honeyeaters. The female builds the small, cup-shaped nest close to the ground in dense shrubs such as tea-tree, Lomandra and wattles or in grass tussocks or new growth from a burnt stump. The nest is made from closely woven grass, bark and spider-web, and is lined with mammal fur or hair. Like a mainland Lichenostomus species, the White-eared Honeyeater, this species is known for its habit of collecting hair from live animals such as horses and dogs and even humans. The female incubates the eggs and feeds the young. She will leave the nestlings to be fed by the male if she is starting a second nest and brood, however the males will drive off the fledglings when they are old enough to disperse (usually for about three weeks, but often till the end of the season) and he will eventually drive the female away as well, once all the young have fledged. This species is parasitised by both Pallid Cuckoos and Fan-tailed Cuckoos.